› 2vtu

X-ray diffraction
3.5Å resolution

crystal structure of bacteriophage MS2 covalent coat protein dimer

Released:
DOI: 10.2210/pdb2vtu/pdb
PDB entry 2vtu coloured by chain, ensemble of 2 models, front view.
PDB entry 2vtu coloured by chain, ensemble of 2 models, side view.
PDB entry 2vtu coloured by chain, ensemble of 2 models, top view.
Source organism: Escherichia phage MS2
Primary publication:
Crystal packing of a bacteriophage MS2 coat protein mutant corresponds to octahedral particles.
Plevka P, Tars K, Liljas L
Protein Sci 17 1731-9 (2008)
PMID: 18662904

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo 48-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Capsid protein Chains: J, L
Molecule details ›
Chains: J, L
Length: 257 amino acids
Theoretical weight: 27.37 KDa
Source organism: Escherichia phage MS2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03612 (Residues: 2-130; Coverage: 99%)
Sequence domains: Levivirus coat protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: F432
Unit cell:
a: 220.438Å b: 220.438Å c: 220.438Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.272 0.272 0.31
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

A novel delivery platform based on Bacteriophage MS2 virus-like particles.
Fu et al. (2016)
4 other articles

5 mentions without citation

Evolution of crystallins for a role in the vertebrate eye lens.
Slingsby et al. (2013)
4 more