› 2vd3

X-ray diffraction
2.45Å resolution

The structure of histidine inhibited HisG from Methanobacterium thermoautotrophicum

Released:
DOI: 10.2210/pdb2vd3/pdb
PDB entry 2vd3 coloured by chain, front view.
PDB entry 2vd3 coloured by chain, side view.
PDB entry 2vd3 coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Entry authors: Lohkamp B, Schweikert T, Lapthorn AJ

Function and Biology Details

Reaction catalysed: 
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127894 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 31.34 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O27550 (Residues: 2-287; Coverage: 100%)
Gene names: MTH_1506, hisG
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand HIS 2 x HIS
Ligand CL 9 x CL
Ligand IMD 3 x IMD
Ligand MG 9 x MG
Ligand MPD 5 x MPD
Ligand MRD 1 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS
Spacegroup: R32
Unit cell:
a: 112.103Å b: 112.103Å c: 247.622Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.226 0.287
Expression system: Escherichia coli BL21

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