› 2v4y

X-ray diffraction
2.8Å resolution

THE STRUCTURE OF E. COLI UMP KINASE IN COMPLEX WITH ITS ALLOSTERIC REGULATOR GTP

Released:
DOI: 10.2210/pdb2v4y/pdb
PDB entry 2v4y coloured by chain, front view.
PDB entry 2v4y coloured by chain, side view.
PDB entry 2v4y coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural and functional characterization of Escherichia coli UMP kinase in complex with its allosteric regulator GTP.
Meyer P, Evrin C, Briozzo P, Joly N, Bârzu O, Gilles AM
J Biol Chem 283 36011-8 (2008)
PMID: 18945668

Function and Biology Details

Reaction catalysed: 
ATP + UMP = ADP + UDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 241 amino acids
Theoretical weight: 26 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7E9 (Residues: 1-241; Coverage: 100%)
Gene names: JW0166, b0171, pyrH, smbA
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

1 bound ligand:
Ligand GTP 12 x GTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 66.28Å b: 145.78Å c: 146.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

13 citation in other articles

Pyrimidine homeostasis is accomplished by directed overflow metabolism.
Reaves et al. (2013)
12 more

2 mentions without citation

Coupling dynamics and evolutionary information with structure to identify protein regulatory and functional binding sites.
Mishra et al. (2019)
1 more