› 2uuw

X-ray diffraction
2.76Å resolution

2.75 angstrom structure of the D347G D348G mutant structure of Sapporo Virus RdRp Polymerase

Released:
DOI: 10.2210/pdb2uuw/pdb
PDB entry 2uuw coloured by chain, front view.
PDB entry 2uuw coloured by chain, side view.
PDB entry 2uuw coloured by chain, top view.
Source organism: Sapporo virus
Entry authors: Fullerton S, Robel I, Schuldt L, Gebhardt J, Tucker P, Rohayem J

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease-polymerase p70 Chain: A
Molecule details ›
Chain: A
Length: 515 amino acids
Theoretical weight: 56.37 KDa
Source organism: Sapporo virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q69014 (Residues: 1135-1649; Coverage: 23%)
Gene name: ORF1
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 58.012Å b: 91.397Å c: 95.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.245 0.287
Expression system: Escherichia coli BL21(DE3)

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