› 2toh

X-ray diffraction
2.3Å resolution

TYROSINE HYDROXYLASE CATALYTIC AND TETRAMERIZATION DOMAINS FROM RAT

Released:
DOI: 10.2210/pdb2toh/pdb
PDB entry 2toh coloured by chain, front view.
PDB entry 2toh coloured by chain, side view.
PDB entry 2toh coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of tyrosine hydroxylase with bound cofactor analogue and iron at 2.3 A resolution: self-hydroxylation of Phe300 and the pterin-binding site.
Goodwill KE, Sabatier C, Stevens RC
Biochemistry 37 13437-45 (1998)
PMID: 9753429

Function and Biology Details

Reaction catalysed: 
L-tyrosine + tetrahydrobiopterin + O(2) = L-dopa + 4a-hydroxytetrahydrobiopterin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137483 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine 3-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 343 amino acids
Theoretical weight: 39.29 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04177 (Residues: 156-498; Coverage: 69%)
Gene name: Th
Sequence domains: Biopterin-dependent aromatic amino acid hydroxylase
Structure domains: Aromatic amino acid hydroxylase

Ligands and Environments


Cofactor: Ligand HBI 1 x HBI
2 bound ligands:
Ligand FE 1 x FE
Ligand CL 1 x CL
1 modified residue:
Ligand MTY 1 x MTY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: F222
Unit cell:
a: 189.464Å b: 148.595Å c: 56.986Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.276
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structural and mechanistic studies on 2-oxoglutarate-dependent oxygenases and related enzymes.
Schofield et al. (1999)
11 more

10 mentions without citation

Mechanism of aromatic amino acid hydroxylation.
Fitzpatrick PF. (2003)
9 more