› 2reu

X-ray diffraction
1.9Å resolution

Crystal Structure of the C-terminal of Sau3AI fragment

Released:
DOI: 10.2210/pdb2reu/pdb
PDB entry 2reu coloured by chain, front view.
PDB entry 2reu coloured by chain, side view.
PDB entry 2reu coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structure and function of C-terminal Sau3AI domain.
Xu CY, Yu F, Xu SJ, Ding Y, Sun LH, Tang L, Hu XJ, Zhang ZH, He JH
Biochim Biophys Acta 1794 118-23 (2009)
PMID: 18930848

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type II restriction enzyme Sau3AI Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 30.39 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P16667 (Residues: 232-489; Coverage: 53%)
Gene name: sau3AIR

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P21212
Unit cell:
a: 76.789Å b: 122.009Å c: 34.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.236
Expression system: Escherichia coli

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Citations

2 mentions without citation

Evolution of the methyl directed mismatch repair system in Escherichia coli.
Putnam CD. (2016)
1 more