› 2rbh

X-ray diffraction
2.1Å resolution

Gamma-glutamyl cyclotransferase

Released:
DOI: 10.2210/pdb2rbh/pdb
PDB entry 2rbh coloured by chain, front view.
PDB entry 2rbh coloured by chain, side view.
PDB entry 2rbh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase. An essential enzyme in the gamma-glutamyl cycle.
Oakley AJ, Yamada T, Liu D, Coggan M, Clark AG, Board PG
J Biol Chem 283 22031-42 (2008)
PMID: 18515354

Function and Biology Details

Reaction catalysed: 
Alpha-(gamma-L-glutamyl)-L-amino acid = alpha-L-amino acid + 5-oxo-L-proline
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130886 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-glutamylcyclotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 188 amino acids
Theoretical weight: 20.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75223 (Residues: 1-188; Coverage: 100%)
Gene names: C7orf24, CRF21, GGCT
Sequence domains: AIG2-like family
Structure domains: Gamma-glutamyl cyclotransferase-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 34.926Å b: 99.406Å c: 99.948Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.201 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Oxidative Stress in Cancer.
Hayes et al. (2020)
13 more

4 mentions without citation

Mammalian proapoptotic factor ChaC1 and its homologues function as γ-glutamyl cyclotransferases acting specifically on glutathione.
Kumar et al. (2012)
3 more