› 2r8r

X-ray diffraction
2.3Å resolution

Crystal structure of the N-terminal region (19..243) of sensor protein KdpD from Pseudomonas syringae pv. tomato str. DC3000

Released:
DOI: 10.2210/pdb2r8r/pdb
PDB entry 2r8r coloured by chain, front view.
PDB entry 2r8r coloured by chain, side view.
PDB entry 2r8r coloured by chain, top view.
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Entry authors: Nocek B, Mulligan R, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183414 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.38 KDa
Source organism: Pseudomonas syringae pv. tomato str. DC3000
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q883V3 (Residues: 19-243; Coverage: 25%)
Gene names: PSPTO_2245, kdpD
Sequence domains: Osmosensitive K+ channel His kinase sensor domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 2 x GOL
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 56.859Å b: 56.859Å c: 372.783Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.216
Expression system: Escherichia coli BL21(DE3)

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