› 2r1j

X-ray diffraction
1.53Å resolution

Crystal Structure of the P22 c2 Repressor protein in complex with the synthetic operator 9T

Released:
DOI: 10.2210/pdb2r1j/pdb
PDB entry 2r1j coloured by chain, front view.
PDB entry 2r1j coloured by chain, side view.
PDB entry 2r1j coloured by chain, top view.
Source organism: Lederbergvirus P22
Primary publication:
P22 c2 repressor-operator complex: mechanisms of direct and indirect readout.
Watkins D, Hsiao C, Woods KK, Koudelka GB, Williams LD
Biochemistry 47 2325-38 (2008)
PMID: 18237194

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115815 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Repressor protein C2 Chains: L, R
Molecule details ›
Chains: L, R
Length: 68 amino acids
Theoretical weight: 7.63 KDa
Source organism: Salmonella virus P22
Expression system: Escherichia coli
UniProt:
  • Canonical: P69202 (Residues: 1-68; Coverage: 32%)
Gene name: C2
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
5'-D(*DCP*DAP*DTP*DTP*DTP*DAP*DAP*DGP*DAP*DTP*DAP*DTP*DCP*DTP*DTP*DAP*DAP*DAP*DTP*DA)-3' Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Lederbergvirus P22
Expression system: Not provided
5'-D(*DTP*DAP*DTP*DTP*DTP*DAP*DAP*DGP*DAP*DTP*DAP*DTP*DCP*DTP*DTP*DAP*DAP*DAP*DTP*DG)-3' Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
Source organism: Lederbergvirus P22
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43
Unit cell:
a: 64.105Å b: 64.105Å c: 101.685Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.225
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
3 more

15 mentions without citation

Evolving insights on how cytosine methylation affects protein-DNA binding.
Dantas Machado et al. (2015)
14 more