› 2r1g

Electron Microscopy
12.5Å resolution

Coordinates of the thermus thermophilus 30S components neighboring RbfA as obtained by fitting into the CRYO-EM map of A 30S-RBFA complex

Released:
DOI: 10.2210/pdb2r1g/pdb
PDB entry 2r1g coloured by chain, front view.
PDB entry 2r1g coloured by chain, side view.
PDB entry 2r1g coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structural aspects of RbfA action during small ribosomal subunit assembly.
Datta PP, Wilson DN, Kawazoe M, Swami NK, Kaminishi T, Sharma MR, Booth TM, Takemoto C, Fucini P, Yokoyama S, Agrawal RK
Mol Cell 28 434-45 (2007)
PMID: 17996707
Related structures: EMD-1413

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158452 (preferred)
Entry contents:
3 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (10 distinct):
Small ribosomal subunit protein uS9 Chain: G
Molecule details ›
Chain: G
Length: 127 amino acids
Theoretical weight: 14.3 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: P62669 (Residues: 2-128; Coverage: 99%)
Gene names: TT_C1100, rps9, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS12 Chain: H
Molecule details ›
Chain: H
Length: 124 amino acids
Theoretical weight: 13.8 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: P61941 (Residues: 2-125; Coverage: 94%)
Gene names: TT_C1333, rps12, rpsL
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: I
Molecule details ›
Chain: I
Length: 125 amino acids
Theoretical weight: 14.21 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: P62655 (Residues: 2-126; Coverage: 99%)
Gene names: TT_C1303, rps13, rpsM
Sequence domains: Ribosomal protein S13/S18
16S RIBOSOMAL RNA HELIX 1 Chain: A
Molecule details ›
Chain: A
Length: 24 nucleotides
Theoretical weight: 7.78 KDa
16S RIBOSOMAL RNA HELIX 18 Chain: B
Molecule details ›
Chain: B
Length: 48 nucleotides
Theoretical weight: 15.54 KDa
16S RIBOSOMAL RNA HELIX 27 Chain: C
Molecule details ›
Chain: C
Length: 29 nucleotides
Theoretical weight: 9.44 KDa
16S RIBOSOMAL RNA HELIX 28 Chain: D
Molecule details ›
Chain: D
Length: 28 nucleotides
Theoretical weight: 8.97 KDa
16S RIBOSOMAL RNA HELIX 44 Chain: E
Molecule details ›
Chain: E
Length: 11 nucleotides
Theoretical weight: 3.46 KDa
16S RIBOSOMAL RNA HELIX 44 Chain: X
Molecule details ›
Chain: X
Length: 11 nucleotides
Theoretical weight: 3.53 KDa
16S RIBOSOMAL RNA HELIX 45 Chain: F
Molecule details ›
Chain: F
Length: 29 nucleotides
Theoretical weight: 9.43 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 12.5Å
Relevant EMDB volumes: EMD-1413
Expression system: Escherichia coli

Quick links

Citations

13 review citations

The universally conserved prokaryotic GTPases.
Verstraeten et al. (2011)
12 more

1 mention without citation

Structural aspects of RbfA action during small ribosomal subunit assembly.
Datta et al. (2007)