› 2qym

X-ray diffraction
1.9Å resolution

crystal structure of unliganded PDE4C2

Released:
DOI: 10.2210/pdb2qym/pdb
PDB entry 2qym coloured by chain, front view.
PDB entry 2qym coloured by chain, side view.
PDB entry 2qym coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the four subfamilies of phosphodiesterase-4 provide insight into the selectivity of their inhibitors.
Wang H, Peng MS, Chen Y, Geng J, Robinson H, Houslay MD, Cai J, Ke H
Biochem J 408 193-201 (2007)
PMID: 17727341

Function and Biology Details

Reaction catalysed: 
Adenosine 3',5'-cyclic phosphate + H(2)O = adenosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170652 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3',5'-cyclic-AMP phosphodiesterase 4C Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 40.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q08493 (Residues: 306-663; Coverage: 50%)
Gene names: DPDE1, PDE4C
Sequence domains: 3'5'-cyclic nucleotide phosphodiesterase
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6122
Unit cell:
a: 74.738Å b: 74.738Å c: 275.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.229
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Advances in targeting cyclic nucleotide phosphodiesterases.
Maurice et al. (2014)
13 more

3 mentions without citation

Structures of the four subfamilies of phosphodiesterase-4 provide insight into the selectivity of their inhibitors.
Wang et al. (2007)
2 more