› 2qxf

X-ray diffraction
1.5Å resolution

Product bound structure of exonuclease I at 1.5 angstrom resolution

Released:
DOI: 10.2210/pdb2qxf/pdb
PDB entry 2qxf coloured by chain, front view.
PDB entry 2qxf coloured by chain, side view.
PDB entry 2qxf coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate.
Busam RD
Acta Crystallogr D Biol Crystallogr 64 206-10 (2008)
PMID: 18219121

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138310 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exodeoxyribonuclease I Chain: A
Molecule details ›
Chain: A
Length: 482 amino acids
Theoretical weight: 55.51 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P04995 (Residues: 1-475; Coverage: 100%)
Gene names: JW1993, b2011, cpeA, sbcB, xonA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand NA 1 x NA
Ligand TMP 1 x TMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 52.63Å b: 91.849Å c: 102.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.224
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Single-stranded heteroduplex intermediates in lambda Red homologous recombination.
Maresca et al. (2010)
4 more