› 2qdc

X-ray diffraction
2Å resolution

Crystal structure of the HePTP catalytic domain D236A mutant

Released:
DOI: 10.2210/pdb2qdc/pdb
PDB entry 2qdc coloured by chain, front view.
PDB entry 2qdc coloured by chain, side view.
PDB entry 2qdc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of substrate recognition by hematopoietic tyrosine phosphatase.
Critton DA, Tortajada A, Stetson G, Peti W, Page R
Biochemistry 47 13336-45 (2008)
PMID: 19053285

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 7 Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 35.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35236 (Residues: 65-360; Coverage: 82%)
Gene name: PTPN7
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P61
Unit cell:
a: 127.258Å b: 127.258Å c: 59.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.163 0.19
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Molecular basis of MAP kinase regulation.
Peti et al. (2013)
4 more

1 mention without citation

Visualizing active-site dynamics in single crystals of HePTP: opening of the WPD loop involves coordinated movement of the E loop.
Critton et al. (2011)