› 2q98

X-ray diffraction
2.7Å resolution

X-ray structure of a prolactin antagonist

Released:
DOI: 10.2210/pdb2q98/pdb
PDB entry 2q98 coloured by chain, front view.
PDB entry 2q98 coloured by chain, side view.
PDB entry 2q98 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and thermodynamic bases for the design of pure prolactin receptor antagonists: X-ray structure of Del1-9-G129R-hPRL.
Jomain JB, Tallet E, Broutin I, Hoos S, van Agthoven J, Ducruix A, Kelly PA, Kragelund BB, England P, Goffin V
J Biol Chem 282 33118-31 (2007)
PMID: 17785459

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Prolactin Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01236 (Residues: 38-227; Coverage: 96%)
Gene name: PRL
Sequence domains: Somatotropin hormone family
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I4
Unit cell:
a: 122.59Å b: 122.59Å c: 28.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.211 0.302
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Electrostatic Interactions in Protein Structure, Folding, Binding, and Condensation.
Zhou et al. (2018)
10 more

3 mentions without citation

Analysis of site-specific histidine protonation in human prolactin.
Tettamanzi et al. (2008)
2 more