2q2e

X-ray diffraction
4Å resolution

Crystal structure of the topoisomerase VI holoenzyme from Methanosarcina mazei

Released:
Source organism: Methanosarcina mazei
Primary publication:
Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI.
Nat Struct Mol Biol 14 611-9 (2007)
PMID: 17603498

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-185804 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type 2 DNA topoisomerase 6 subunit A Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 42.18 KDa
Source organism: Methanosarcina mazei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PUB7 (Residues: 1-369; Coverage: 100%)
Gene names: MM_2418, top6A
Sequence domains:
Structure domains:
Type 2 DNA topoisomerase 6 subunit B Chain: B

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P6422
Unit cell:
a: 227.812Å b: 227.812Å c: 208.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.308 0.306 0.349
Expression system: Escherichia coli