› 2pwh

X-ray diffraction
2Å resolution

Crystal structure of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45

Released:
DOI: 10.2210/pdb2pwh/pdb
PDB entry 2pwh coloured by chain, front view.
PDB entry 2pwh coloured by chain, side view.
PDB entry 2pwh coloured by chain, top view.
Source organism: Burkholderia ubonensis subsp. mesacidophila
Primary publication:
Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization.
Ravaud S, Robert X, Watzlawick H, Haser R, Mattes R, Aghajari N
J Biol Chem 282 28126-36 (2007)
PMID: 17597061

Function and Biology Details

Reaction catalysed: 
L-threo-3-methylaspartate = L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 556 amino acids
Theoretical weight: 63.81 KDa
Source organism: Burkholderia ubonensis subsp. mesacidophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2PS28 (Residues: 29-584; Coverage: 99%)
Gene name: mutB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P1
Unit cell:
a: 63.58Å b: 71.54Å c: 81.75Å
α: 67.66° β: 73.04° γ: 70.44°
R-values:
R R work R free
0.172 0.172 0.215
Expression system: Escherichia coli

Quick links

Citations

5 review citations

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
4 more

1 mention without citation

Characterization of Halomonas sp. strain H11 α-glucosidase activated by monovalent cations and its application for efficient synthesis of α-D-glucosylglycerol.
Ojima et al. (2012)