› 2pjo

X-ray diffraction
1.8Å resolution

Ricin a-chain (recombinant) complex with n-methylurea

Released:
DOI: 10.2210/pdb2pjo/pdb
PDB entry 2pjo coloured by chain, front view.
PDB entry 2pjo coloured by chain, side view.
PDB entry 2pjo coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site.
Carra JH, McHugh CA, Mulligan S, Machiesky LM, Soares AS, Millard CB
BMC Struct Biol 7 72 (2007)
PMID: 17986339

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 30.07 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02879 (Residues: 36-302; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand NMU 1 x NMU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P41212
Unit cell:
a: 67.73Å b: 67.73Å c: 140.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.247
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Immunity to ricin: fundamental insights into toxin-antibody interactions.
O'Hara et al. (2012)
10 other articles

3 mentions without citation

Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site.
Carra et al. (2007)
2 more