› 2pbr

X-ray diffraction
1.96Å resolution

Crystal structure of thymidylate kinase (aq_969) from Aquifex Aeolicus VF5

Released:
DOI: 10.2210/pdb2pbr/pdb
PDB entry 2pbr coloured by chain, front view.
PDB entry 2pbr coloured by chain, side view.
PDB entry 2pbr coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Entry authors: Jeyakanthan J, Kanaujia SP, Vasuki Ranjani C, Sekar K, Nakagawa N, Ebihara A, Kuramitsu S, Shinkai A, Shiro Y, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + dTMP = ADP + dTDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.4 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67099 (Residues: 1-195; Coverage: 100%)
Gene names: aq_969, tmk
Sequence domains: Thymidylate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P1
Unit cell:
a: 42.765Å b: 50.478Å c: 52.823Å
α: 95.19° β: 90.81° γ: 111.68°
R-values:
R R work R free
0.194 0.194 0.242
Expression system: Escherichia coli

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