› 2pak

X-ray diffraction
2.4Å resolution

Structure of a H51N mutant dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase from Aneurinibacillus thermoaerophilus complexed with TDP

Released:
DOI: 10.2210/pdb2pak/pdb
PDB entry 2pak coloured by chain, front view.
PDB entry 2pak coloured by chain, side view.
PDB entry 2pak coloured by chain, top view.
Source organism: Aneurinibacillus thermoaerophilus
Primary publication:
The x-ray structure of dTDP-4-keto-6-deoxy-D-glucose-3,4-ketoisomerase.
Davis ML, Thoden JB, Holden HM
J Biol Chem 282 19227-36 (2007)
PMID: 17459872

Function and Biology Details

Reaction catalysed: 
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose = dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TDP-4-oxo-6-deoxy-alpha-D-glucose-3,4-oxoisomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 16.23 KDa
Source organism: Aneurinibacillus thermoaerophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6T1W8 (Residues: 1-139; Coverage: 100%)
Gene name: fdtA
Sequence domains: WxcM-like, C-terminal
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:
Ligand TYD 2 x TYD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 61.3Å b: 61.3Å c: 201.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.207 0.295
Expression system: Escherichia coli

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Citations

3 review citations

Natural-product sugar biosynthesis and enzymatic glycodiversification.
Thibodeaux et al. (2008)
2 more