› 2p7c

Solution NMR

Solution structure of the bacillus licheniformis BlaI monomeric form in complex with the blaP half-operator.

Released:
DOI: 10.2210/pdb2p7c/pdb
PDB entry 2p7c coloured by chain, ensemble of 10 models, front view.
PDB entry 2p7c coloured by chain, ensemble of 10 models, side view.
PDB entry 2p7c coloured by chain, ensemble of 10 models, top view.
Source organism: Bacillus licheniformis
Primary publication:
Conformational and thermodynamic changes of the repressor/DNA operator complex upon monomerization shed new light on regulation mechanisms of bacterial resistance against beta-lactam antibiotics.
Boudet J, Duval V, Van Melckebeke H, Blackledge M, Amoroso A, Joris B, Simorre JP
Nucleic Acids Res 35 4384-95 (2007)
PMID: 17576674

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115756 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Penicillinase repressor Chain: B
Molecule details ›
Chain: B
Length: 82 amino acids
Theoretical weight: 9.51 KDa
Source organism: Bacillus licheniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06555 (Residues: 1-82; Coverage: 64%)
Gene names: blaI, penI
Sequence domains: Penicillinase repressor
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Strand 1 of Twelve base-pair DNA Chain: A
Molecule details ›
Chain: A
Length: 12 nucleotides
Theoretical weight: 3.67 KDa
Source organism: Bacillus licheniformis
Expression system: Not provided
Strand 2 of Twelve base-pair DNA Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
Source organism: Bacillus licheniformis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing from randomized coordinates followed by restrained molecular dynamics.
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 citation in other articles

A peptidoglycan fragment triggers β-lactam resistance in Bacillus licheniformis.
Amoroso et al. (2012)
2 more

2 mentions without citation

Solution structure and DNA-binding properties of the winged helix domain of the meiotic recombination HOP2 protein.
Moktan et al. (2014)
1 more