› 2p1z

X-ray diffraction
2.44Å resolution

Crystal structure of phosphoribosyltransferase from Corynebacterium diphtheriae

Released:
DOI: 10.2210/pdb2p1z/pdb
PDB entry 2p1z coloured by chain, front view.
PDB entry 2p1z coloured by chain, side view.
PDB entry 2p1z coloured by chain, top view.
Source organism: Corynebacterium diphtheriae NCTC 13129
Entry authors: Chang C, Li H, Clancy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180029 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Orotate phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 19.3 KDa
Source organism: Corynebacterium diphtheriae NCTC 13129
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6NF12 (Residues: 1-177; Coverage: 100%)
Gene names: DIP2097, pyrE
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 72.438Å b: 103.709Å c: 39.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.226 0.308
Expression system: Escherichia coli

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