› 2oxp

X-ray diffraction
2Å resolution

Crystal Structure of Staphylococcal Nuclease mutant V66D/P117G/H124L/S128A

Released:
DOI: 10.2210/pdb2oxp/pdb
PDB entry 2oxp coloured by chain, front view.
PDB entry 2oxp coloured by chain, side view.
PDB entry 2oxp coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
High apparent dielectric constant inside a protein reflects structural reorganization coupled to the ionization of an internal Asp.
Karp DA, Gittis AG, Stahley MR, Fitch CA, Stites WE, García-Moreno E B
Biophys J 92 2041-53 (2007)
PMID: 17172297

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thermonuclease Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.78 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NXI6 (Residues: 80-228; Coverage: 73%)
Gene names: MW0769, nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

1 bound ligand:
Ligand THP 1 x THP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P41
Unit cell:
a: 47.579Å b: 47.579Å c: 62.791Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.234
Expression system: Escherichia coli

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Citations

9 review citations

Chemistry of Hofmeister anions and osmolytes.
Zhang et al. (2010)
8 more

7 mentions without citation

Role of flexibility and polarity as determinants of the hydration of internal cavities and pockets in proteins.
Damjanović et al. (2007)
6 more