› 2oxb

X-ray diffraction
2.6Å resolution

Crystal structure of a cell-wall invertase (E203Q) from Arabidopsis thaliana in complex with sucrose

Released:
DOI: 10.2210/pdb2oxb/pdb
PDB entry 2oxb coloured by chain, front view.
PDB entry 2oxb coloured by chain, side view.
PDB entry 2oxb coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
An alternate sucrose binding mode in the E203Q Arabidopsis invertase mutant: an X-ray crystallography and docking study.
Mátrai J, Lammens W, Jonckheer A, Le Roy K, Rabijns A, Van den Ende W, De Maeyer M
Proteins 71 552-64 (2008)
PMID: 17963237

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-fructofuranosidase, insoluble isoenzyme CWINV1 Chain: A
Molecule details ›
Chain: A
Length: 537 amino acids
Theoretical weight: 61.1 KDa
Source organism: Arabidopsis thaliana
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q43866 (Residues: 48-584; Coverage: 97%)
Gene names: At3g13790, BFRUCT1, CWINV1, MMM17.26
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P32
Unit cell:
a: 106.454Å b: 106.454Å c: 50.548Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.205 0.205 0.268
Expression system: Komagataella pastoris

Quick links

Citations

3 review citations

Structural insights into glycoside hydrolase family 32 and 68 enzymes: functional implications.
Lammens et al. (2009)
2 more

1 mention without citation

Crystal structures of Aspergillus japonicus fructosyltransferase complex with donor/acceptor substrates reveal complete subsites in the active site for catalysis.
Chuankhayan et al. (2010)