› 2ouy

X-ray diffraction
1.9Å resolution

crystal structure of pde10a2 mutant D564A in complex with cAMP.

Released:
DOI: 10.2210/pdb2ouy/pdb
PDB entry 2ouy coloured by chain, front view.
PDB entry 2ouy coloured by chain, side view.
PDB entry 2ouy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insight into substrate specificity of phosphodiesterase 10.
Wang H, Liu Y, Hou J, Zheng M, Robinson H, Ke H
Proc Natl Acad Sci U S A 104 5782-7 (2007)
PMID: 17389385

Function and Biology Details

Reaction catalysed: 
Nucleoside 3',5'-cyclic phosphate + H(2)O = nucleoside 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195088 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A Chains: A, B
Molecule details ›
Chains: A, B
Length: 331 amino acids
Theoretical weight: 38.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Y233 (Residues: 715-1042; Coverage: 31%)
Gene name: PDE10A
Structure domains: 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand MG 2 x MG
Ligand CMP 1 x CMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 49.391Å b: 82.266Å c: 155.931Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Mammalian cyclic nucleotide phosphodiesterases: molecular mechanisms and physiological functions.
Francis et al. (2011)
6 more

2 mentions without citation

Structural insight into substrate specificity of phosphodiesterase 10.
Wang et al. (2007)
1 more