› 2or1

X-ray diffraction
2.5Å resolution

RECOGNITION OF A DNA OPERATOR BY THE REPRESSOR OF PHAGE 434. A VIEW AT HIGH RESOLUTION

Released:
DOI: 10.2210/pdb2or1/pdb
PDB entry 2or1 coloured by chain, front view.
PDB entry 2or1 coloured by chain, side view.
PDB entry 2or1 coloured by chain, top view.
Source organism: Phage 434
Primary publication:
Recognition of a DNA operator by the repressor of phage 434: a view at high resolution.
Aggarwal AK, Rodgers DW, Drottar M, Ptashne M, Harrison SC
Science 242 899-907 (1988)
PMID: 3187531

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115737 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Repressor protein CI Chains: L, R
Molecule details ›
Chains: L, R
Length: 69 amino acids
Theoretical weight: 7.56 KDa
Source organism: Phage 434
UniProt:
  • Canonical: P16117 (Residues: 2-70; Coverage: 73%)
Gene name: CI
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*AP*GP*TP*AP*CP*AP*AP*AP*CP*TP*TP*TP*CP*TP*TP*G P*TP*AP*T)-3') Chain: A
Molecule details ›
Chain: A
Length: 20 nucleotides
Theoretical weight: 6.11 KDa
Source organism: Phage 434
Expression system: Not provided
DNA (5'-D(*TP*AP*TP*AP*CP*AP*AP*GP*AP*AP*AP*GP*TP*TP*TP*GP*T P*AP*CP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.16 KDa
Source organism: Phage 434
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 148.3Å b: 65Å c: 27.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 not available not available
Expression system: Not provided

Quick links

Citations

37 review citations

A structural taxonomy of DNA-binding domains.
Harrison SC. (1991)
36 more

24 mentions without citation

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
23 more