› 2okw

X-ray diffraction
1.9Å resolution

A non-invasive GFP-based biosensor for mercury ions

Released:
DOI: 10.2210/pdb2okw/pdb
PDB entry 2okw coloured by chain, front view.
PDB entry 2okw coloured by chain, side view.
PDB entry 2okw coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake.
Chapleau RR, Blomberg R, Ford PC, Sagermann M
Protein Sci 17 614-22 (2008)
PMID: 18305194

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 236 amino acids
Theoretical weight: 26.9 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 6 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 88.178Å b: 128.843Å c: 147.889Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.268
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.
Greenwald et al. (2018)
4 more

1 mention without citation

Structural basis for a hand-like site in the calcium sensor CatchER with fast kinetics.
Zhang et al. (2013)