› 2ohx

X-ray diffraction
1.8Å resolution

REFINED CRYSTAL STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE-NADH COMPLEX AT 1.8 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb2ohx/pdb
PDB entry 2ohx coloured by chain, front view.
PDB entry 2ohx coloured by chain, side view.
PDB entry 2ohx coloured by chain, top view.
Source organism: Equus caballus
Primary publication:
Refined crystal structure of liver alcohol dehydrogenase-NADH complex at 1.8 A resolution.
Al-Karadaghi S, Cedergren-Zeppezauer ES, Hövmoller S
Acta Crystallogr D Biol Crystallogr 50 793-807 (1994)
PMID: 15299346

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alcohol dehydrogenase E chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.85 KDa
Source organism: Equus caballus
Expression system: Not provided
UniProt:
  • Canonical: P00327 (Residues: 2-375; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand ZN 4 x ZN
Ligand DMS 2 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 51.76Å b: 44.54Å c: 94.61Å
α: 104.8° β: 102.3° γ: 70.6°
R-values:
R R work R free
0.173 0.173 not available
Expression system: Not provided

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Citations

20 citation in other articles

A structurally conserved water molecule in Rossmann dinucleotide-binding domains.
Bottoms et al. (2002)
19 more

13 mentions without citation

A novel and efficient tool for locating and characterizing protein cavities and binding sites.
Tripathi et al. (2010)
12 more