› 2o8x

X-ray diffraction
3Å resolution

Crystal structure of the "-35 element" promoter recognition domain of Mycobacterium tuberculosis SigC

Released:
DOI: 10.2210/pdb2o8x/pdb
PDB entry 2o8x coloured by chain, front view.
PDB entry 2o8x coloured by chain, side view.
PDB entry 2o8x coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural and biophysical studies on two promoter recognition domains of the extra-cytoplasmic function sigma factor sigma(C) from Mycobacterium tuberculosis.
Thakur KG, Joshi AM, Gopal B
J Biol Chem 282 4711-4718 (2007)
PMID: 17145760

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo 36-mer
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-161476 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ECF RNA polymerase sigma factor SigC Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 70 amino acids
Theoretical weight: 7.39 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGH1 (Residues: 117-185; Coverage: 37%)
Gene names: MTCY49.08, Rv2069, sigC
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: F23
Unit cell:
a: 161.33Å b: 161.33Å c: 161.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.271
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The sigma factors of Mycobacterium tuberculosis: regulation of the regulators.
Sachdeva et al. (2010)
3 more

4 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
3 more