› 2o8t

X-ray diffraction
1.45Å resolution

Crystal Structure and Binding Epitopes of Urokinase-type Plasminogen Activator (C122A/N145Q) in complex with Inhibitors

Released:
DOI: 10.2210/pdb2o8t/pdb
PDB entry 2o8t coloured by chain, front view.
PDB entry 2o8t coloured by chain, side view.
PDB entry 2o8t coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Zhao G, Yuan C, Jiang L, Huang Z, Huang M

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133249 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urokinase-type plasminogen activator chain B Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.44 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00749 (Residues: 179-431; Coverage: 62%)
Gene name: PLAU
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand 1PE 2 x 1PE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: R3
Unit cell:
a: 120.91Å b: 120.91Å c: 42.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.242 0.265
Expression system: Komagataella pastoris

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