› 2o7s

X-ray diffraction
1.78Å resolution

Crystal Structure of the A. thaliana DHQ-dehydroshikimate-SDH-shikimate-NADP(H)

Released:
DOI: 10.2210/pdb2o7s/pdb
PDB entry 2o7s coloured by chain, front view.
PDB entry 2o7s coloured by chain, side view.
PDB entry 2o7s coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
The DHQ-dehydroshikimate-SDH-shikimate-NADP(H) Complex: Insights into Metabolite Transfer in the Shikimate Pathway
Singh SA, Christendat D
Cryst Growth Des 7 2153-2160 (2007)

Function and Biology Details

Reactions catalysed: 
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
3-dehydroquinate = 3-dehydroshikimate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-193505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 523 amino acids
Theoretical weight: 57.12 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9SQT8 (Residues: 90-603; Coverage: 85%)
Gene names: At3g06350, EMB3004, F24P17.18
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
3 bound ligands:
Ligand SO4 1 x SO4
Ligand DHK 2 x DHK
Ligand TLA 1 x TLA
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P3221
Unit cell:
a: 97.447Å b: 97.447Å c: 116.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.269 not available 0.233
Expression system: Escherichia coli

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