› 2o4e

Solution NMR

The solution structure of a protein-protein interaction module from a family 84 glycoside hydrolase of Clostridium perfringens

Released:
DOI: 10.2210/pdb2o4e/pdb
PDB entry 2o4e coloured by chain, ensemble of 20 models, front view.
PDB entry 2o4e coloured by chain, ensemble of 20 models, side view.
PDB entry 2o4e coloured by chain, ensemble of 20 models, top view.
Source organism: Clostridium perfringens
Primary publication:
Three-dimensional structure of a putative non-cellulosomal cohesin module from a Clostridium perfringens family 84 glycoside hydrolase.
Chitayat S, Gregg K, Adams JJ, Ficko-Blean E, Bayer EA, Boraston AB, Smith SP
J Mol Biol 375 20-8 (2008)
PMID: 17999932

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-GlcNAcase NagJ Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 17.44 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0TR53 (Residues: 768-909; Coverage: 15%)
Gene names: CPF_1442, nagJ
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 93%
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Insights into cellulosome assembly and dynamics: from dissection to reconstruction of the supramolecular enzyme complex.
Smith et al. (2013)
1 more

1 mention without citation

Portrait of an enzyme, a complete structural analysis of a multimodular {beta}-N-acetylglucosaminidase from Clostridium perfringens.
Ficko-Blean et al. (2009)