› 2nwc

X-ray diffraction
3.02Å resolution

A 3.02 angstrom crystal structure of wild-type apo GroEL in a monoclinic space group

Released:
DOI: 10.2210/pdb2nwc/pdb
PDB entry 2nwc coloured by chain, front view.
PDB entry 2nwc coloured by chain, side view.
PDB entry 2nwc coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Purification, crystallization and structure determination of native GroEL from Escherichia coli lacking bound potassium ions.
Kiser PD, Lodowski DT, Palczewski K
Acta Crystallogr Sect F Struct Biol Cryst Commun 63 457-61 (2007)
PMID: 17554162

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 547 amino acids
Theoretical weight: 57.26 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6F5 (Residues: 2-548; Coverage: 100%)
Gene names: JW4103, b4143, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 136.423Å b: 262.254Å c: 147.074Å
α: 90° β: 99.83° γ: 90°
R-values:
R R work R free
0.228 0.227 0.263
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Comparative analysis of the Photorhabdus luminescens and the Yersinia enterocolitica genomes: uncovering candidate genes involved in insect pathogenicity.
Heermann et al. (2008)
2 other articles

6 mentions without citation

UCSF Chimera, MODELLER, and IMP: an integrated modeling system.
Yang et al. (2012)
5 more