› 2nsf

X-ray diffraction
1.75Å resolution

Crystal structure of the mycothiol-dependent maleylpyruvate isomerase

Released:
DOI: 10.2210/pdb2nsf/pdb
PDB entry 2nsf coloured by chain, front view.
PDB entry 2nsf coloured by chain, side view.
PDB entry 2nsf coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Primary publication:
Crystal structures and site-directed mutagenesis of a mycothiol-dependent enzyme reveal a novel folding and molecular basis for mycothiol-mediated maleylpyruvate isomerization.
Wang R, Yin YJ, Wang F, Li M, Feng J, Zhang HM, Zhang JP, Liu SJ, Chang WR
J Biol Chem 282 16288-294 (2007)
PMID: 17428791

Function and Biology Details

Reaction catalysed: 
3-maleylpyruvate = 3-fumarylpyruvate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypothetical protein Cgl3021 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.65 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8NLC1 (Residues: 1-241; Coverage: 100%)
Gene name: Cgl3021
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand ZN 1 x ZN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3221
Unit cell:
a: 67.266Å b: 67.266Å c: 128.245Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Biosynthesis and functions of mycothiol, the unique protective thiol of Actinobacteria.
Newton et al. (2008)
3 more

4 mentions without citation

Novel L-cysteine-dependent maleylpyruvate isomerase in the gentisate pathway of Paenibacillus sp. strain NyZ101.
Liu et al. (2012)
3 more