› 2ns7

X-ray diffraction
2.4Å resolution

How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor

Released:
DOI: 10.2210/pdb2ns7/pdb
PDB entry 2ns7 coloured by chain, front view.
PDB entry 2ns7 coloured by chain, side view.
PDB entry 2ns7 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor.
Luckner SR, Klotzsche M, Berens C, Hillen W, Muller YA
J Mol Biol 368 780-90 (2007)
PMID: 17374541

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class B from transposon Tn10 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 208 amino acids
Theoretical weight: 23.53 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P04483 (Residues: 1-201; Coverage: 97%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6122
Unit cell:
a: 107.91Å b: 107.91Å c: 303.94Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.23 0.277
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metal sensor proteins: nature's metalloregulated allosteric switches.
Giedroc et al. (2007)
2 more

3 mentions without citation

Rigid substructure search.
Shirvanyants et al. (2011)
2 more