› 2lgf

Solution NMR

Solution structure of Ca2+/calmodulin complexed with a peptide representing the calmodulin-binding domain of L-selectin

Released:
DOI: 10.2210/pdb2lgf/pdb
PDB entry 2lgf coloured by chain, front view.
PDB entry 2lgf coloured by chain, side view.
PDB entry 2lgf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into calmodulin-regulated L-selectin ectodomain shedding.
Gifford JL, Ishida H, Vogel HJ
J Biol Chem 287 26513-27 (2012)
PMID: 22711531

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143912 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 3-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
L-selectin Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.83 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P14151 (Residues: 349-363; Coverage: 4%)
Gene names: LNHR, LYAM1, SELL

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 40%
Refinement method: torsion angle dynamics, simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Structural diversity of calmodulin binding to its target sites.
Tidow et al. (2013)
4 more

5 mentions without citation

Structural insights into calmodulin-regulated L-selectin ectodomain shedding.
Gifford et al. (2012)
4 more