› 2kue

Solution NMR

NMR structure of the PASTA domain 2 and 3 of Mycobacterium tuberculosis of PknB

Released:
DOI: 10.2210/pdb2kue/pdb
PDB entry 2kue coloured by chain, ensemble of 30 models, front view.
PDB entry 2kue coloured by chain, ensemble of 30 models, side view.
PDB entry 2kue coloured by chain, ensemble of 30 models, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The structure of PknB extracellular PASTA domain from mycobacterium tuberculosis suggests a ligand-dependent kinase activation.
Barthe P, Mukamolova GV, Roumestand C, Cohen-Gonsaud M
Structure 18 606-15 (2010)
PMID: 20462494

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknB Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 14.24 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WI81 (Residues: 423-557; Coverage: 22%)
Gene names: MTCY10H4.14c, Rv0014c, pknB
Sequence domains: PASTA domain
Structure domains: Trypsin Inhibitor V; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 84%
Refinement method: restrained molecular dynamics in a hydrated environment
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Eukaryote-like serine/threonine kinases and phosphatases in bacteria.
Pereira et al. (2011)
15 more

3 mentions without citation

Mycobacterium tuberculosis Serine/Threonine Protein Kinases.
Prisic et al. (2014)
2 more