› 2kq4

Solid-state NMR

Atomic resolution protein structure determination by three-dimensional transferred echo double resonance solid-state nuclear magnetic resonance spectroscopy

Released:
DOI: 10.2210/pdb2kq4/pdb
PDB entry 2kq4 coloured by chain, ensemble of 10 models, front view.
PDB entry 2kq4 coloured by chain, ensemble of 10 models, side view.
PDB entry 2kq4 coloured by chain, ensemble of 10 models, top view.
Source organism: Streptococcus sp. 'group G'
Primary publication:
Atomic resolution protein structure determination by three-dimensional transferred echo double resonance solid-state nuclear magnetic resonance spectroscopy.
Nieuwkoop AJ, Wylie BJ, Franks WT, Shah GJ, Rienstra CM
J Chem Phys 131 095101 (2009)
PMID: 19739873

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chain: X
Molecule details ›
Chain: X
Length: 56 amino acids
Theoretical weight: 6.23 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 303-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DGSA-distance geometry simulated annealing, simulated annealing
Expression system: Escherichia coli

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Citations

3 review citations

Protein structure determination by magic-angle spinning solid-state NMR, and insights into the formation, structure, and stability of amyloid fibrils.
Comellas et al. (2013)
2 more

2 mentions without citation

Supramolecular protein structure determination by site-specific long-range intermolecular solid state NMR spectroscopy.
Nieuwkoop et al. (2010)
1 more