› 2k9f

Solution NMR

Structural features of the complex between the DsbD N-terminal and the PilB N-terminal domains from Neisseria meningitidis

Released:
DOI: 10.2210/pdb2k9f/pdb
PDB entry 2k9f coloured by chain, ensemble of 10 models, front view.
PDB entry 2k9f coloured by chain, ensemble of 10 models, side view.
PDB entry 2k9f coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
Formation of the complex between DsbD and PilB N-terminal domains from Neisseria meningitidis necessitates an adaptability of nDsbD.
Quinternet M, Tsan P, Selme-Roussel L, Jacob C, Boschi-Muller S, Branlant G, Cung MT
Structure 17 1024-33 (2009)
PMID: 19604482

Function and Biology Details

Reactions catalysed: 
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
Peptide-L-methionine + thioredoxin disulfide + H(2)O = peptide-L-methionine (R)-S-oxide + thioredoxin
Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191669 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptide methionine sulfoxide reductase MsrA/MsrB Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 15.85 KDa
Source organism: Neisseria meningitidis serogroup A
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JWM8 (Residues: 34-176; Coverage: 27%)
Gene names: NMA0290, msrAB, pilB
Sequence domains: Redoxin
Structure domains: Glutaredoxin
Thiol:disulfide interchange protein DsbD Chain: B
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.17 KDa
Source organism: Neisseria meningitidis serogroup B
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9JYM0 (Residues: 19-146; Coverage: 22%)
Gene names: NMB1519, dsbD
Sequence domains: Thiol:disulfide interchange protein DsbD, N-terminal
Structure domains: Thiol:disulfide interchange protein DsbD, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Immunoglobulin domains in Escherichia coli and other enterobacteria: from pathogenesis to applications in antibody technologies.
Bodelón et al. (2013)
1 more

1 mention without citation

Structural plasticity of the thioredoxin recognition site of yeast methionine S-sulfoxide reductase Mxr1.
Ma et al. (2011)