› 2ju4

Solution NMR

NMR structure of the gamma subunit of cGMP phosphodiesterase

Released:
DOI: 10.2210/pdb2ju4/pdb
PDB entry 2ju4 coloured by chain, ensemble of 100 models, front view.
PDB entry 2ju4 coloured by chain, ensemble of 100 models, side view.
PDB entry 2ju4 coloured by chain, ensemble of 100 models, top view.
Source organism: Bos taurus
Primary publication:
Intrinsically disordered gamma-subunit of cGMP phosphodiesterase encodes functionally relevant transient secondary and tertiary structure.
Song J, Guo LW, Muradov H, Artemyev NO, Ruoho AE, Markley JL
Proc Natl Acad Sci U S A 105 1505-10 (2008)
PMID: 18230733

Function and Biology Details

Reaction catalysed: 
Guanosine 3',5'-cyclic phosphate + H(2)O = guanosine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma Chain: A
Molecule details ›
Chain: A
Length: 87 amino acids
Theoretical weight: 9.47 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04972 (Residues: 1-87; Coverage: 100%)
Gene names: PDE6G, PDEG
Sequence domains: Retinal cGMP phosphodiesterase, gamma subunit
Structure domains: Retinal cGMP phosphodiesterase, gamma subunit

Ligands and Environments

1 bound ligand:
Ligand RCY 10 x RCY
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 68%
Refinement method: molecular dynamics, simulated annealing
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Linking folding and binding.
Wright et al. (2009)
11 more

3 mentions without citation

Photoreceptor phosphodiesterase (PDE6): activation and inactivation mechanisms during visual transduction in rods and cones.
Cote RH. (2021)
2 more