› 2jhs

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF RHOGDI K135H,K138H,K141H MUTANT

Released:
DOI: 10.2210/pdb2jhs/pdb
PDB entry 2jhs coloured by chain, front view.
PDB entry 2jhs coloured by chain, side view.
PDB entry 2jhs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Protein crystallization by surface entropy reduction: optimization of the SER strategy.
Cooper DR, Boczek T, Grelewska K, Pinkowska M, Sikorska M, Zawadzki M, Derewenda Z
Acta Crystallogr D Biol Crystallogr 63 636-45 (2007)
PMID: 17452789

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156565 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho GDP-dissociation inhibitor 1 Chain: A
Molecule details ›
Chain: A
Length: 138 amino acids
Theoretical weight: 15.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52565 (Residues: 67-202; Coverage: 67%)
Gene names: ARHGDIA, GDIA1
Sequence domains: RHO protein GDP dissociation inhibitor
Structure domains: Coagulation Factor XIII, subunit A, domain 1

Ligands and Environments

1 bound ligand:
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P3221
Unit cell:
a: 75.316Å b: 75.316Å c: 91.283Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.178 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins.
Koide et al. (2009)
14 more

1 mention without citation

MR-REX: molecular replacement by cooperative conformational search and occupancy optimization on low-accuracy protein models.
Virtanen et al. (2018)