› 2jfa

X-ray diffraction
2.55Å resolution

ESTROGEN RECEPTOR ALPHA LBD IN COMPLEX WITH AN AFFINITY-SELECTED COREPRESSOR PEPTIDE

Released:
DOI: 10.2210/pdb2jfa/pdb
PDB entry 2jfa coloured by chain, front view.
PDB entry 2jfa coloured by chain, side view.
PDB entry 2jfa coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into corepressor recognition by antagonist-bound estrogen receptors.
Heldring N, Pawson T, McDonnell D, Treuter E, Gustafsson JA, Pike AC
J Biol Chem 282 10449-55 (2007)
PMID: 17283072

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136906 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Estrogen receptor Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 28.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03372 (Residues: 303-533; Coverage: 39%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Estrogen receptor Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 28.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03372 (Residues: 303-533; Coverage: 39%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
COREPRESSOR PEPTIDE Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 16 amino acids
Theoretical weight: 1.9 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand RAL 2 x RAL
Ligand SO4 4 x SO4
1 modified residue:
Ligand CCS 1 x CCS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 126.573Å b: 126.573Å c: 113.429Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.193 0.213
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

14 review citations

Molecular mechanism of estrogen-estrogen receptor signaling.
Yaşar et al. (2017)
13 more

12 mentions without citation

Versatility or promiscuity: the estrogen receptors, control of ligand selectivity and an update on subtype selective ligands.
Ng et al. (2014)
11 more