› 2j4j

X-ray diffraction
2.1Å resolution

Crystal structure of uridylate kinase from Sulfolobus solfataricus in complex with UMP and AMPPCP to 2.1 Angstrom resolution

Released:
DOI: 10.2210/pdb2j4j/pdb
PDB entry 2j4j coloured by chain, front view.
PDB entry 2j4j coloured by chain, side view.
PDB entry 2j4j coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Structural and enzymatic investigation of the Sulfolobus solfataricus uridylate kinase shows competitive UTP inhibition and the lack of GTP stimulation.
Jensen KS, Johansson E, Jensen KF
Biochemistry 46 2745-57 (2007)
PMID: 17297917

Function and Biology Details

Reaction catalysed: 
ATP + UMP = ADP + UDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 226 amino acids
Theoretical weight: 25.02 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97ZE2 (Residues: 2-227; Coverage: 100%)
Gene names: SSO0976, pyrH
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

5 bound ligands:
Ligand U5P 5 x U5P
Ligand ACP 5 x ACP
Ligand CO 6 x CO
Ligand MG 2 x MG
Ligand 4TC 1 x 4TC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 86.174Å b: 126.438Å c: 134.991Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.215 0.242
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

The structural basis of ATP as an allosteric modulator.
Lu et al. (2014)
12 more