› 2is9

X-ray diffraction
1.92Å resolution

Structure of yeast DCN-1

Released:
DOI: 10.2210/pdb2is9/pdb
PDB entry 2is9 coloured by chain, front view.
PDB entry 2is9 coloured by chain, side view.
PDB entry 2is9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for the function of DCN-1 in protein Neddylation.
Yang X, Zhou J, Sun L, Wei Z, Gao J, Gong W, Xu RM, Rao Z, Liu Y
J Biol Chem 282 24490-4 (2007)
PMID: 17597076

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-171350 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Defective in cullin neddylation protein 1 Chain: A
Molecule details ›
Chain: A
Length: 204 amino acids
Theoretical weight: 24.61 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12395 (Residues: 66-269; Coverage: 76%)
Gene names: DCN1, L3111, YLR128W
Sequence domains: Cullin binding
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PT 1 x PT
Ligand CL 4 x CL
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I41
Unit cell:
a: 81.107Å b: 81.107Å c: 106.023Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Function and regulation of protein neddylation. 'Protein modifications: beyond the usual suspects' review series.
Rabut et al. (2008)
7 more