› 2idr

X-ray diffraction
1.85Å resolution

Crystal structure of translation initiation factor EIF4E from wheat

Released:
DOI: 10.2210/pdb2idr/pdb
PDB entry 2idr coloured by chain, front view.
PDB entry 2idr coloured by chain, side view.
PDB entry 2idr coloured by chain, top view.
Source organism: Triticum aestivum
Primary publication:
The structure of eukaryotic translation initiation factor-4E from wheat reveals a novel disulfide bond.
Monzingo AF, Dhaliwal S, Dutt-Chaudhuri A, Lyon A, Sadow JH, Hoffman DW, Robertus JD, Browning KS
Plant Physiol 143 1504-18 (2007)
PMID: 17322339

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eukaryotic translation initiation factor 4E-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 177 amino acids
Theoretical weight: 20.24 KDa
Source organism: Triticum aestivum
Expression system: Escherichia coli
UniProt:
  • Canonical: P29557 (Residues: 39-215; Coverage: 82%)
Gene names: TRAES_3BF151900060CFD_c1, eIF4E
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P61
Unit cell:
a: 66.337Å b: 66.337Å c: 180.853Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.211 0.234
Expression system: Escherichia coli

Quick links

Citations

6 review citations

eIF4E: new family members, new binding partners, new roles.
Rhoads RE. (2009)
5 more

12 mentions without citation

Mutational analysis of plant cap-binding protein eIF4E reveals key amino acids involved in biochemical functions and potyvirus infection.
German-Retana et al. (2008)
11 more