› 2i88

X-ray diffraction
2.5Å resolution

Crystal structure of the Channel-forming Domain of Colicin E1

Released:
DOI: 10.2210/pdb2i88/pdb
PDB entry 2i88 coloured by chain, front view.
PDB entry 2i88 coloured by chain, side view.
PDB entry 2i88 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A mechanism for toxin insertion into membranes is suggested by the crystal structure of the channel-forming domain of colicin E1.
Elkins P, Bunker A, Cramer WA, Stauffacher CV
Structure 5 443-58 (1997)
PMID: 9083117

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136444 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-E1 Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P02978 (Residues: 332-522; Coverage: 37%)
Gene name: cea
Sequence domains: Colicin pore forming domain
Structure domains: Colicin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: I4
Unit cell:
a: 87.46Å b: 87.46Å c: 59.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.172 0.284
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Colicin biology.
Cascales et al. (2007)
23 more

9 mentions without citation

Structural Basis of the Pore-Forming Toxin/Membrane Interaction.
Li et al. (2021)
8 more