› 2i7s

X-ray diffraction
1.35Å resolution

Crystal structure of Re(phen)(CO)3 (Thr124His)(His83Gln) Azurin Cu(II) from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb2i7s/pdb
PDB entry 2i7s coloured by chain, front view.
PDB entry 2i7s coloured by chain, side view.
PDB entry 2i7s coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Relaxation dynamics of Pseudomonas aeruginosa Re(I)(CO)3(alpha-diimine)(HisX)+ (X = 83, 107, 109, 124, 126)Cu(II) azurins.
Blanco-Rodríguez AM, Busby M, Ronayne K, Towrie M, Grădinaru C, Sudhamsu J, Sýkora J, Hof M, Zális S, Di Bilio AJ, Crane BR, Gray HB, Vlcek A
J Am Chem Soc 131 11788-800 (2009)
PMID: 19639996

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132373 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.99 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 4 x CU
Ligand REP 4 x REP
Ligand CON 1 x CON
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P1
Unit cell:
a: 33.135Å b: 58.98Å c: 66.107Å
α: 82.92° β: 78.11° γ: 76.68°
R-values:
R R work R free
0.205 0.205 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Proton-coupled electron transfer.
Huynh et al. (2007)
1 more

4 mentions without citation

Trends in coordination of rhenium organometallic complexes in the Protein Data Bank.
Brink et al. (2022)
3 more