› 2i6y

X-ray diffraction
2.5Å resolution

Structure and Mechanism of Mycobacterium tuberculosis Salicylate Synthase, MbtI

Released:
DOI: 10.2210/pdb2i6y/pdb
PDB entry 2i6y coloured by chain, front view.
PDB entry 2i6y coloured by chain, side view.
PDB entry 2i6y coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure and mechanism of MbtI, the salicylate synthase from Mycobacterium tuberculosis.
Zwahlen J, Kolappan S, Zhou R, Kisker C, Tonge PJ
Biochemistry 46 954-64 (2007)
PMID: 17240979

Function and Biology Details

Reactions catalysed: 
Isochorismate = salicylate + pyruvate
Chorismate = isochorismate
Chorismate = prephenate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Salicylate synthase Chain: A
Molecule details ›
Chain: A
Length: 470 amino acids
Theoretical weight: 50.95 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFX1 (Residues: 2-450; Coverage: 100%)
Gene names: Rv2386c, mbtI, trpE2
Sequence domains: chorismate binding enzyme
Structure domains: Anthranilate synthase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: C2221
Unit cell:
a: 49.206Å b: 143.603Å c: 123.759Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.232 0.294
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Biosynthesis of mycobacterial lipids by polyketide synthases and beyond.
Quadri LE. (2014)
7 more

1 mention without citation

Pericyclic reactions catalyzed by chorismate-utilizing enzymes.
Lamb AL. (2011)