› 2i5r

X-ray diffraction
1.65Å resolution

Structure of small Toprim domain-containing protein from B. stearothermophilus in complex with Mg2+

Released:
DOI: 10.2210/pdb2i5r/pdb
PDB entry 2i5r coloured by chain, front view.
PDB entry 2i5r coloured by chain, side view.
PDB entry 2i5r coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Crystal structure and putative function of small Toprim domain-containing protein from Bacillus stearothermophilus.
Rezácová P, Borek D, Moy SF, Joachimiak A, Otwinowski Z
Proteins 70 311-9 (2008)
PMID: 17705269

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177358 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toprim domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 122 amino acids
Theoretical weight: 13.99 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KVJ9 (Residues: 1-119; Coverage: 100%)
Gene name: GK3002
Sequence domains: Toprim domain
Structure domains: Dna Topoisomerase Vi A Subunit; Chain: A, domain 2

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand GOL 6 x GOL
Ligand SO4 2 x SO4
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 36.667Å b: 73.031Å c: 114.922Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Topoisomerases and site-specific recombinases: similarities in structure and mechanism.
Yang W. (2010)
15 other articles

1 mention without citation

Crystal structure and putative function of small Toprim domain-containing protein from Bacillus stearothermophilus.
Rezácová et al. (2008)