› 2hnk

X-ray diffraction
2.3Å resolution

Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans

Released:
DOI: 10.2210/pdb2hnk/pdb
PDB entry 2hnk coloured by chain, front view.
PDB entry 2hnk coloured by chain, side view.
PDB entry 2hnk coloured by chain, top view.
Source organism: Leptospira interrogans
Primary publication:
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans.
Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W
J Struct Biol 159 523-8 (2007)
PMID: 17561415

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SAM-dependent O-methyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 239 amino acids
Theoretical weight: 26.86 KDa
Source organism: Leptospira interrogans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8F8Y3 (Residues: 1-231; Coverage: 100%)
Gene name: LA_0415
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

3 bound ligands:
Ligand SO4 4 x SO4
Ligand SAH 3 x SAH
Ligand PEG 7 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P2221
Unit cell:
a: 157.81Å b: 60.331Å c: 138.232Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.243
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.
Parveen et al. (2011)
3 more

4 mentions without citation

Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.
Lashley et al. (2022)
3 more