› 2hmw

X-ray diffraction
3Å resolution

Square-shaped octameric ring structure of an RCK domain with ATP bound

Released:
DOI: 10.2210/pdb2hmw/pdb
PDB entry 2hmw coloured by chain, front view.
PDB entry 2hmw coloured by chain, side view.
PDB entry 2hmw coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The RCK domain of the KtrAB K+ transporter: multiple conformations of an octameric ring.
Albright RA, Ibar JL, Kim CU, Gruner SM, Morais-Cabral JH
Cell 126 1147-59 (2006)
PMID: 16990138

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ktr system potassium uptake protein A Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.08 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32080 (Residues: 1-144; Coverage: 65%)
Gene names: BSU31090, ktrA, yuaA
Sequence domains: TrkA-N domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I422
Unit cell:
a: 126.28Å b: 126.28Å c: 98.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.264 0.303
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Molecular mechanisms of BK channel activation.
Cui et al. (2009)
8 more

5 mentions without citation

Structure of potassium channels.
Kuang et al. (2015)
4 more